Chicken Infectious Bursal Disease Ab Rapid Detection Card(colloidal gold method) Wholesaler

Chicken Infectious Bursal Disease Ab Rapid Detection Card

[Intended use]

Infectious bursa of Fabricius (IBD), also known as Gamblo disease, is an acute and highly infectious disease caused by infectious bursa of Fabricius virus. Due to its sudden onset, short course, high mortality rate, and the ability to cause immune suppression in chickens, this disease remains one of the main infectious diseases in the chicken industry. Through the joint efforts of scientific research departments and a large number of veterinary workers, the disease has been effectively controlled to a certain extent.

This reagent is used to detect infectious bursa of Fabricius virus antibody in poultry serum, and can be used for immune effect evaluation, auxiliary diagnosis, etc.

[Principle]

This reagent is made based on the principle of competitive colloidal gold immunochromatography (GICA), and has good sensitivity to different subtypes of infectious bursa of Fabricius virus antibodies. After the sample is added to the sample well, it moves along the chromatographic membrane with IBD antigen and colloidal gold markers. If there is IBD antibody in the sample, it competes with the colloidal gold marker for IBD antigen, so that the detection line does not show purple red. If there is no IBD antibody in the sample, a color reaction occurs.

[Kit composition]

No.	Name	40T / box
1.	test cards (including straws)	40
2.	Buffer	1bottle
3.	instruction manual	1

[Storage and expiry date] Store in a cool and dry place (2-30 ° C), valid for 24 months.

[Sample preparation]

Serum: Take 2-3ml of blood according to the conventional method and place it in a clean and dry test tube. Let it stand for about 1 hour. After the blood is coagulated, centrifuge at 4,000 rpm for 10 minutes. (You can also leave the blood for about 2 hours and let the blood coagulate naturally. Serum), and isolate the serum. Require serum to be clear, no hemolysis, no pollution. Serum samples can be stored at 2-8 ° C in the short-term and at -20 ° C in the long-term.

Whole blood: Whole blood with anticoagulant can be used as the test sample (whole blood samples cannot be frozen).

【experiment method】

1. Tear off the aluminum foil packaging bag of the test card, remove the test card, and place it on a flat, clean surface.

2. Use the matching pipette to suck the prepared sample, and add 1 drop (about 25ul) slowly and slowly into the sample well (at this time, no liquid flows out of the observation well).

3. After waiting for 10 minutes, add two drops (about 60ul) of buffer solution to the sample well using a dropper bottle containing buffer solution.

4. Leave at room temperature for 10-15 minutes to judge the results. Results longer than 30 minutes are invalid.

Note that adding too much sample or buffer will give incorrect results.